Determination of the Tumorsphere Formation Efficiency (TFE) with the PromoCell Cancer Stem Cell Medium

The PromoCell Cancer Stem Cell Medium has been designed to meet your requirements for the extended serial 3D tumorsphere culture. This Cancer Stem Cell Medium supports the most commonly used cancer stem cell lines in tumorsphere/mammosphere culture. In contrast to the current adherent 2D culture of cancer cells, this type of 3D culture selectively exploits inherent biologic features of Cancer Stem Cells (CSC), such as anoikis resistance and self-renewal (Fig. 3). Continuous proliferation is also supported during serial passage of 3D tumorsphere cultures (Fig. 2). Thus, this culture system is also applicable for in vitro models of metastasis. PromoCell’s Cancer Stem Cell Medium is ready-to-use and defined, providing a standardized culture devoid of stimuli of uncharacterized origin. This is a significant benefit in terms of CSC which are a population of highly responsive stem cells requiring reliable and reproducible control of the selfrenewal/differentiation axis. The defined PromoCell Cancer Stem Cell Medium is suitable for the cost-efficient and standardized routine culture of tumorspheres/mammospheres for a wide range of cell lines (Tab. 1).